Chandler-Loop-System
ready to use system platform

Chandler Loop System ®

Apart from clinical research, the chandler loop method gains in importance for the testing of medical devices, especially the biocompatibility of their surfaces and coatings. Up-to-date information:   https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6054932/



ISO 10993-4 describes Chandler-Loop tests for the following categories:





Evaluation Procedure

Determination

Test Principle 





Thrombosis

scanning electron microscopy SEM

platelet adhesion and aggregation, leukocyte adhesion, fibrinogen adsorbtion

microscopy





 

Coagulation

 

marker for thrombin generation

thrombin-antithrombin-III complex TAT

ELISA





Platelets

number of platelets

blood cell counting

cell counter micros 60






marker for platelet activaton

ß-thromboglobin

ELISA

Haematology

number of white and red blood cells

blood cell counting            leukocytes, erythrocytes, Hb, Hk

cell counter micros 60





 

products of leucocyte activat.

PMN-Elastase

ELISA





 Haemolysis

 

free Plasma hemoglobin

PFHb Level

colorimetric assay





Complement system

marker for activation of the C3 complement factor

C3a

ELISA






marker for activation of the terminal complement complex

SC5b-9

ELISA